Author's response to reviews Title: High Quality Copy Number and Genotype Data from FFPE Samples Using Molecular Inversion Probe (MIP) Microarrays Authors:
نویسندگان
چکیده
Yuker Wang ([email protected]) Victoria E.H. Carlton ([email protected]) George Karlin-Neumann ([email protected]) Ronald Sapolsky ([email protected]) Li Zhang ([email protected]) Martin Moorhead ([email protected]) Zhigang C Wang ([email protected]) Andrea L Richardson ([email protected]) Robert Warren ([email protected]) Axel Walther ([email protected]) Melissa Bondy ([email protected]) Aysegul Sahin ([email protected]) Ralf Krahe ([email protected]) Musaffe Tuna ([email protected]) Patricia A Thompson ([email protected]) Paul T Spellman ([email protected]) Joe W Gray ([email protected]) Gordon B Mills ([email protected]) Malek Faham ([email protected])
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Allele quantification using molecular inversion probes (MIP)
Detection of genomic copy number changes has been an important research area, especially in cancer. Several high-throughput technologies have been developed to detect these changes. Features that are important for the utility of technologies assessing copy number changes include the ability to interrogate regions of interest at the desired density as well as the ability to differentiate the two...
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The molecular inversion probe (MIP) assay technology was originally developed for single nucleotide polymorphism (SNP) genotyping, but has subsequently been used for identifying other types of genetic variation including focal insertions and deletions, larger copy number alterations (CNAs), loss of heterozygosity (LOH), and most recently, for somatic mutation detection. The assay requires as li...
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Array comparative hybridization has been used successfully to identify genomic alterations in stillbirth material; however, high DNA quantity and quality requirements may limit its utility in some fetal samples. Molecular inversion probe (MIP) array analysis of FFPE stillbirth autopsy samples circumvents the challenges associated with karyotype and short-term fetal cell culture, requires limite...
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Formalin-fixed, paraffin-embedded (FFPE) material tends to yield degraded DNA and is thus suboptimal for use in many downstream applications. We describe an integrated analysis of genotype, loss of heterozygosity (LOH), and copy number for DNA derived from FFPE tissues using oligonucleotide microarrays containing over 500K single nucleotide polymorphisms. A prequalifying PCR test predicted the ...
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